Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum
نویسندگان
چکیده
منابع مشابه
Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum.
Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified ...
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Supplemental assays, such as recombinant immunoblot assays (RIBA), are used to confirm detection of antibodies to hepatitis C virus (HCV). However, due to their expense, they are not widely used in developing countries. The purpose of our study was to compare the results of second- and third-generation (G2 and G3, respectively) enzyme immunoassays (EIAs) and to resolve discordant results by usi...
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Departamento de Clínica Médica, Universidade Federal de Mato Grosso, Rua L s/ no, 78070-000 Cuiabá, MT, Brasil *Fundação Nacional de Saúde, Av. Getúlio Vargas 867, 78045-720 Cuiabá, MT, Brasil **Departamento de Virologia, Instituto Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil ***Hospital Prof. Edgar Santos, Universidade Federal da Bahia, Rua João das Botas s/ no, 40110-16...
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Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HAV HRP conjugate; in the second (COMP-2), test sera and anti-HAV HRP conjugate competed for HAV epitopes previously adsorbed to anti-HAV IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a ...
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The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2016
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cvi.00028-16